Long non-coding RNA SPRY4-IT1 promotes cell proliferation and invasion by regulation of Cdc20 in pancreatic cancer cells.

Long non-coding RNA SPRY4-IT1 promotes cell proliferation and invasion by regulation of Cdc20 in pancreatic cancer cells.

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Accumulating evidence has demonstrated that long non-coding RNAs (lncRNAs) play a critical role in the development la rams crop top of human cancers including pancreatic cancer.Long non-coding RNA SPRY4-IT1 (sprouty4-intron transcript 1) has been reported to play an oncogenic role in various types of human carcinomas.However, the role of SPRY4-IT1 in pancreatic cancer is unclear.The objective of this study was to determine the function of SPRY4-IT1 on proliferation and invasion in pancreatic cancer.

In the current study, we dissected the function and mechanism of SPRY4-IT1 by multiple approaches including Real-time RT-PCR, Western blotting analysis, MTT assay, Wound healing assay, Transwell assay, and transfection.We found that down-regulation of SPRY4-IT1 inhibited cell growth and induced cell apoptosis in pancreatic cancer cells.Moreover, SPRY4-IT1 knockdown induced cell cycle arrest at G0/G1 phase.Furthermore, inhibition of SPRY4-IT1 retarded cell migration and invasion in pancreatic cancer cells.

Overexpression of SPRY4-IT1 enhanced cell growth and invasion, and inhibited cell apoptosis in pancreatic cancer cells.Mechanistically, suppression of SPRY4-IT1 inhibited camo iphone se case the expression of Cdc20 in pancreatic cancer cells.Our findings demonstrated that inhibition of SPRY4-IT1 could be a potential therapeutic approach for the treatment of pancreatic cancer.